PODXL antibody (Extracellular Domain)

Catalog No. ABIN6940326

This anti-PODXL antibody is a Mouse Monoclonal antibody detecting PODXL in WB, IHC, IP, FACS, IF and StM. Suitable for Human, Rat and Rabbit.

Quick Overview for PODXL antibody (Extracellular Domain) (ABIN6940326)

Target: PODXL (Podocalyxin-Like (PODXL))

Reactivity: Human, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This PODXL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Clone: 3D3

Product Details anti-PODXL Antibody

Binding Specificity: Extracellular Domain

Purification: Purified by Protein A/G

Immunogen: A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: PODXL

Background: Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Molecular Weight: 165-170kDa

Gene ID: 5420

UniProt: O00592

Pathways: Tube Formation