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POMC antibody (AA 1-24)

The Mouse Monoclonal anti-POMC antibody has been validated for ELISA, IHC, FACS, IF, Coat, StM and ISt. It is suitable to detect POMC in samples from Human, Mouse and Rat.
Catalog No. ABIN6940335

Quick Overview for POMC antibody (AA 1-24) (ABIN6940335)

Target

See all POMC Antibodies
POMC (Proopiomelanocortin (POMC))

Reactivity

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Human, Mouse, Rat

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This POMC antibody is un-conjugated

Application

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ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Staining Methods (StM), Immunostaining (ISt)

Clone

SPM333
  • Binding Specificity

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    AA 1-24

    Specificity

    ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH), does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. '

    Cross-Reactivity (Details)

    Expected to show a broad species reactivity.

    Purification

    Purified by Protein A/G

    Immunogen

    Synthetic peptide corresponding to aa1-24 of human ACTH

    Isotype

    IgG1 kappa
  • Application Notes

    Positive Control: Normal pituitary gland or pituitary tumor.

    Known Application: ELISA (For coating, order Ab without BSA), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for staining of formalin-fixed tissues)Optimal dilution for a specific application should be determined.

    Restrictions

    For Research Use only
  • Concentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Expiry Date

    24 months
  • Target

    POMC (Proopiomelanocortin (POMC))

    Alternative Name

    POMC

    Molecular Weight

    ACTH is ~5kDa, and the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.

    Gene ID

    5443

    UniProt

    P01189

    Pathways

    Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour
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