KRT20 antibody (AA 196-323)

Catalog No. ABIN6940347

The Mouse Monoclonal anti-KRT20 antibody has been validated for WB, IHC and StM. It is suitable to detect KRT20 in samples from Human.

Quick Overview for KRT20 antibody (AA 196-323) (ABIN6940347)

Target: KRT20 (Keratin 20 (KRT20))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: KRT20-1992

Product Details anti-KRT20 Antibody

Binding Specificity: AA 196-323

Specificity: This MAb recognizes an intermediate filament protein of 46 kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: HT29 cells. Colon Carcinoma.

Known Application: Western Blot (0.5 μg/mL-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: KRT20

Molecular Weight: 46kDa

Gene ID: 54474

UniProt: P35900