POU2AF1 antibody (AA 148-255)

Catalog No. ABIN6940353

The Mouse Monoclonal anti-POU2AF1 antibody has been validated for WB, IHC, FACS and StM. It is suitable to detect POU2AF1 in samples from Human.

Quick Overview for POU2AF1 antibody (AA 148-255) (ABIN6940353)

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POU2AF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Clone: BOB1-2421

Product Details anti-POU2AF1 Antibody

Binding Specificity: AA 148-255

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: Raji, Ramos cells. Spleen or Tonsil.

Known Application: Flow Cytometry (1-2 μg/mL),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for POU2AF1

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Alternative Name: POU2AF1

Background: BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.

Molecular Weight: 35kDa

Gene ID: 5450

UniProt: Q16633