MAML3 antibody

Catalog No. ABIN6940389

The Mouse Monoclonal anti-MAML3 antibody has been validated for ISt, IHC and StM. It is suitable to detect MAML3 in samples from Human.

Quick Overview for MAML3 antibody (ABIN6940389)

Target: MAML3 (Mastermind-Like 3 (MAML3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAML3 antibody is un-conjugated

Application: Immunostaining (ISt), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: MAML3-1303

Product Details anti-MAML3 Antibody

Purification: Purified by Protein A/G

Immunogen: Recombinant human MAML3 protein fragment

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MAML3

Target: MAML3 (Mastermind-Like 3 (MAML3))

Alternative Name: MAML3

Background: MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis.

Molecular Weight: 150-170kDa

Gene ID: 55534

UniProt: Q96JK9