ACPP antibody

Catalog No. ABIN6940393

This Mouse Monoclonal antibody specifically detects ACPP in IHC, IF, FACS and StM. It exhibits reactivity toward Human.

Quick Overview for ACPP antibody (ABIN6940393)

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACPP antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: PASE-4LJ

Product Details anti-ACPP Antibody

Specificity: Recognizes a protein of 52 kDa, identified as prostate specific acid phosphatase (PSAP). This enzyme catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland.PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.

Purification: Purified by Protein A/G

Immunogen: Prostatic acid phosphatase Purified from human seminal plasma

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: PC12 cells. Normal prostate or prostate carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Alternative Name: ACPP

Molecular Weight: 52kDa

Gene ID: 55

UniProt: P15309

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process