BCL2L1 antibody (AA 3-14)

Catalog No. ABIN6940509

The Mouse Monoclonal anti-BCL2L1 antibody has been validated for WB, IHC and StM. It is suitable to detect BCL2L1 in samples from Human, Mouse, Rat and Pig.

Quick Overview for BCL2L1 antibody (AA 3-14) (ABIN6940509)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivity: Human, Mouse, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCL2L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: BX006

Product Details anti-BCL2L1 Antibody

Binding Specificity: AA 3-14

Specificity: Recognizes a protein of 27 kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.

Purification: Purified by Protein A/G

Immunogen: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein.

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.

Known Application: Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Alternative Name: BCL2L1

Molecular Weight: 27kDa

Gene ID: 598

UniProt: Q07817

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling