Recombinant s100a4 antibody (AA 1-200)

Catalog No. ABIN6940545

The Rabbit Monoclonal anti-s100a4 antibody has been validated for WB, IHC, IF, FACS and StM. It is suitable to detect s100a4 in samples from Human and Mouse.

Quick Overview for Recombinant s100a4 antibody (AA 1-200) (ABIN6940545)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This s100a4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: S100A4-2750R

Product Details anti-s100a4 Antibody

Binding Specificity: AA 1-200

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human S100A4 protein (around aa1-200) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Positive Control: HeLa, A549 or A375 cells. Placenta.

Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4

Background: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Molecular Weight: 10-12kDa

Gene ID: 6275

UniProt: P26447

Pathways: S100 Proteins