Recombinant SOX2 antibody (AA 176-305)

Catalog No. ABIN6940614

This anti-SOX2 antibody is a Mouse Monoclonal antibody detecting SOX2 in ELISA, IHC, Coat and StM. Suitable for Human and Mouse.

Quick Overview for Recombinant SOX2 antibody (AA 176-305) (ABIN6940614)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Clone: RSOX2-1792

Product Details anti-SOX2 Antibody

Binding Specificity: AA 176-305

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: MCF-7 cells. Lung or Cervical carcinoma.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2

Background: SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Molecular Weight: 34kDa

Gene ID: 6657

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis