SPTA1 antibody (AA 356-475)

Catalog No. ABIN6940644

This anti-SPTA1 antibody is a Mouse Monoclonal antibody detecting SPTA1 in WB, IHC, ELISA, Coat and StM. Suitable for Human.

Quick Overview for SPTA1 antibody (AA 356-475) (ABIN6940644)

Target: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPTA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Clone: SPTA1-1832

Product Details anti-SPTA1 Antibody

Binding Specificity: AA 356-475

Purification: Purified by Protein A/G

Immunogen: Recombinant human SPTA fragment (around aa356-475) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: K562, HeLa, Jurkat cells. Placenta or Tonsil.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (0.5-2 μg/mL). Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SPTA1

Target: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Alternative Name: SPTA1

Background: Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

Molecular Weight: 240kDa

Gene ID: 6708

UniProt: P02549

Pathways: Regulation of Actin Filament Polymerization