Spectrin beta Chain, Erythrocyte (SPTB) antibody

Catalog No. ABIN6940649

This Mouse Monoclonal antibody specifically detects in WB, IF, IHC, ELISA, Coat and StM. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Spectrin beta Chain, Erythrocyte (SPTB) antibody (ABIN6940649)

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Clone: RG-26

Product Details

Purification: Purified by Protein A/G

Immunogen: Red cell membranes

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Alternative Name: SPTB

Background: Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

Molecular Weight: 246kDa

Gene ID: 6710

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization