CD147 antibody

Catalog No. ABIN6940674

This Mouse Monoclonal antibody specifically detects CD147 in FACS, IHC, IF, AGG and StM. It exhibits reactivity toward Human.

Quick Overview for CD147 antibody (ABIN6940674)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Aggregation (AGG), Staining Methods (StM)

Clone: 8D6

Product Details anti-CD147 Antibody

Specificity: This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Purification: Purified by Protein A/G

Immunogen: Human T cell line Molt 13

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HSB2 cells. Renal Cell, Ovarian carcinoma or Melanoma.

Known Application: Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, and Migation through Matrigel (Order Ab without BSA/Azide),Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1:100-1:200), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: BSG

Molecular Weight: 35kDa (non-reduced), 40kDa (reduced)

Gene ID: 682

UniProt: P35613

Pathways: S100 Proteins