Recombinant GRP94 antibody

Catalog No. ABIN6940801

The Rabbit Monoclonal anti-GRP94 antibody has been validated for IHC, FACS and StM. It is suitable to detect GRP94 in samples from Human, Mouse, Rat, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Sheep, Horse and Xenopus laevis.

Quick Overview for Recombinant GRP94 antibody (ABIN6940801)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Sheep, Horse, Xenopus laevis

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Clone: HSP90B1-3168R

Product Details anti-GRP94 Antibody

Specificity: Recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human HSP90B1 protein

Isotype: IgG

Application Details

Application Notes:

Positive Control: HepG2, U20S cells. About 50 % of breast tumors express grp94 protein.

Known Application: Flow Cytometry (0.5-1 μg/million cells),Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: HSP90B1

Molecular Weight: 94kDa

Gene ID: 7184

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades