Ezrin antibody

Catalog No. ABIN6940877

The Mouse Monoclonal anti-Ezrin antibody has been validated for WB, IHC and StM. It is suitable to detect Ezrin in samples from Human.

Quick Overview for Ezrin antibody (ABIN6940877)

Target: Ezrin (EZR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: CPTC-Ezrin-1

Product Details anti-Ezrin Antibody

Purification: Purified by Protein A/G

Immunogen: Recombinant human full-length Ezrin protein

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: SKBr3, HeLa, HEK293, MCF-7, Jurkat, A431, MCF-7. Cerebral cortex and hippocampus.

Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin

Background: Ezrin, Moesin and Radixin belong to a family of highly homologous actin-associated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70 % homology with Ezrin and are co-expressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation. Overexpression of Ezrin predicts the poor prognosis of gastric adenocarcinoma.

Molecular Weight: 87kDa

Gene ID: 7430

UniProt: P15311

Pathways: Maintenance of Protein Location