Recombinant Caldesmon antibody

Catalog No. ABIN6940942

This anti-Caldesmon antibody is a Rabbit Monoclonal antibody detecting Caldesmon in IHC and StM. Suitable for Human and Rat.

Quick Overview for Recombinant Caldesmon antibody (ABIN6940942)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Caldesmon antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: CALD1-1424R

Product Details anti-Caldesmon Antibody

Specificity: Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Purification: Purified by Protein A/G

Immunogen: Recombinant human full-length CALD1 protein

Isotype: IgG

Application Details

Application Notes:

Positive Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Alternative Name: CALD1

Molecular Weight: 150kDa

Gene ID: 800

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction