ABCC3 antibody (AA 815-957)

Catalog No. ABIN6940972

This anti-ABCC3 antibody is a Mouse Monoclonal antibody detecting ABCC3 in IHC and StM. Suitable for Human.

Quick Overview for ABCC3 antibody (AA 815-957) (ABIN6940972)

Target: ABCC3 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 3 (ABCC3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ABCC3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: ABCC3-2971

Product Details anti-ABCC3 Antibody

Binding Specificity: AA 815-957

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 815-957) of human MRP3 (ABCC3) protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Pancreas.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ABCC3

Target: ABCC3 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 3 (ABCC3))

Alternative Name: ABCC3

Background: The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the MDR1 P-glycoprotein and the multidrug-resistance protein MRP1. MRP1 is an integral membrane protein that contains an MDR-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood. The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter) and MRP3, which are both conjugate export pumps exp- ressed predominantly in hepatocytes. MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells. Conversely, MRP3 localizes to the basolateral membrane where it also medi- ates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane. MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired.

Molecular Weight: 169/137/55/32/65kDa

Gene ID: 8714

UniProt: O15438