CD3 epsilon antibody

Catalog No. ABIN6941023

The Mouse Monoclonal anti-CD3 epsilon antibody is suitable to detect CD3 epsilon in samples from Human and Chimpanzee. It has been validated for FACS, IF, ELISA, Coat and IHC (af).

Quick Overview for CD3 epsilon antibody (ABIN6941023)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Chimpanzee

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat), Immunohistochemistry (Acetone-fixed) (IHC (af))

Clone: UCHT1

Product Details anti-CD3 epsilon Antibody

Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Purification: Purified by Protein A/G

Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Human peripheral blood. Jurkat cells. Tonsil or lymph node.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL). ,Immunohistochemistry (Acetone-fixed frozen tissues) (1-2 μg/mL for 30 minutes at RT),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Molecular Weight: 20kDa

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway