CD5 antibody

Catalog No. ABIN6941054

The Mouse Monoclonal anti-CD5 antibody (Clone CRIS-1) (ABIN6941054) specifically detects CD5 in FACS, IF, IP and Func. The antibody is reactive with Human and Rhesus Monkey samples.

Quick Overview for CD5 antibody (ABIN6941054)

Target: CD5

Reactivity: Human, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Functional Studies (Func)

Clone: CRIS-1

Product Details anti-CD5 Antibody

Specificity: Recognizes a 67 kDa transmembrane protein, which is identified as CD5 (HLDA I, WS Code T 29HLDA III, WS Code T 530). The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms.

Purification: Purified by Protein A/G

Immunogen: Stimulated human leukocytes

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: 293T, Ramos or MOLT-4 cells. Tonsil.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Functional Studies (Order Ab without BSA & Azide), Immunoprecipitation (0.5-1 μg/500 μg protein lysate), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD5

Target: CD5

Alternative Name: CD5

Molecular Weight: 67kDa

Gene ID: 921

UniProt: P06127