CD27 antibody

Catalog No. ABIN6941124

The Mouse Monoclonal anti-CD27 antibody has been validated for FACS, IHC, ELISA, IF, StM and Coat. It is suitable to detect CD27 in samples from Human.

Quick Overview for CD27 antibody (ABIN6941124)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD27 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Staining Methods (StM), Coating (Coat)

Clone: LPFS2-1611

Product Details anti-CD27 Antibody

Specificity: Recognizes a protein of a disulfide-linked 120 kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human CD27 protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Ramos cells. Human peripheral blood lymphocytes. Ramos and lymph node cell lysates. Human tonsil and stomach tissues.

Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Molecular Weight: 120kDa

Gene ID: 939

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints