Recombinant CD44 antibody

Catalog No. ABIN6941191

The Mouse Monoclonal anti-CD44 antibody has been validated for IHC and StM. It is suitable to detect CD44 in samples from Human.

Quick Overview for Recombinant CD44 antibody (ABIN6941191)

Target: CD44

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: RCD44v4-1219

Product Details anti-CD44 Antibody

Specificity: This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 Molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA.The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment corresponding to the v4 domain of human CD44

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: Cervix or Tongue Squamous Cell Carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD44

Target: CD44

Alternative Name: CD44

Molecular Weight: 80-95kDa

Gene ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling