CD70 antibody

Catalog No. ABIN6941250

The Mouse Monoclonal anti-CD70 antibody is suitable to detect CD70 in samples from Human. It has been validated for ELISA, FACS, IP, IF, IHC (fro), Coat and ProA.

Quick Overview for CD70 antibody (ABIN6941250)

Target: CD70 (CD70 Molecule (CD70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD70 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Coating (Coat), Proliferation Assay (ProA)

Clone: BU69

Product Details anti-CD70 Antibody

Specificity: It recognizes a protein of 30 kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This MAb blocks the interaction between CD27 and CD70, and has been shown toinhibit T cell proliferation induced by dendritic cells.

Purification: Purified by Protein A/G

Immunogen: Human WM-1 (Waldenstrom s macroglobulinemia) cell line

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Raji cells. Lymph node or tonsil.

Known Application: ELISA (For coating, order Ab without BSA), ,Inhibits T cell proliferation induced by dendritic cells (Order Ab without Azide), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunoprecipitation (1-2 μg/500 μg protein lysate), ,Immunohistochemistry (Frozen) (0.5-1 μg/mL for 30 minutes at RT),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10mM PBS with 0.05% BSA & 0.05% azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD70

Target: CD70 (CD70 Molecule (CD70))

Alternative Name: CD70

Molecular Weight: 30kDa

Gene ID: 970

UniProt: P32970

Pathways: Cancer Immune Checkpoints