Recombinant MVP antibody

Catalog No. ABIN6941288

This Rabbit Monoclonal antibody specifically detects MVP in IHC and StM. It exhibits reactivity toward Human.

Quick Overview for Recombinant MVP antibody (ABIN6941288)

Target: MVP (Major Vault Protein (MVP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MVP antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: VP2897R

Product Details anti-MVP Antibody

Specificity: Recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human MVP protein

Isotype: IgG

Application Details

Application Notes:

Positive Control: MCF-7/HeLa, breast tumors.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MVP

Target: MVP (Major Vault Protein (MVP))

Alternative Name: MVP

Molecular Weight: 104-110kDa

Gene ID: 9961

UniProt: Q14764