CD3 epsilon antibody

Catalog No. ABIN6941302

The Hamster Monoclonal anti-CD3 epsilon antibody has been validated for FACS, IF and Func. It is suitable to detect CD3 epsilon in samples from Mouse.

Quick Overview for CD3 epsilon antibody (ABIN6941302)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: 145-2C11

Product Details anti-CD3 epsilon Antibody

No Cross-Reactivity: Human

Purification: Purified by Protein A/G

Immunogen: H2Kb specific Mouse cytotoxic T lymphocyte clone BM10 37

Isotype: IgG kappa

Application Details

Application Notes:

Positive Control: MPBMC's or Thymus or Lymph Node.

Known Application: Functional Studies (Order Antibody without BSA & Azide),Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (0.5-1 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: Cd3e

Background: CD3 chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful forin vitroblocking and activation assays, as well as apoptosis induction andin vivoT cell depletion.

Molecular Weight: 23kDa

Gene ID: 12501

UniProt: P22646

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway