toxA antibody

Catalog No. ABIN6941319

The Mouse Monoclonal anti-toxA antibody has been validated for ELISA, IF and Coat. It is suitable to detect toxA in samples from Clostridium difficile.

Quick Overview for toxA antibody (ABIN6941319)

Target: toxA (Clostridium Difficile Toxin A (toxA))

Reactivity: Clostridium difficile

Host: Mouse

Clonality: Monoclonal

Conjugate: This toxA antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Coating (Coat)

Clone: 253-422

Product Details anti-toxA Antibody

Specificity: This MAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.

Purification: Purified by Protein A/G

Immunogen: C. difficile Toxin A

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Clostridium difficile extract or infected cells. Tissue.

Known Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA),Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for toxA

Target: toxA (Clostridium Difficile Toxin A (toxA))

Alternative Name: Clostridium difficile (Toxin A)

Molecular Weight: 308kDa (Toxin A)

UniProt: P16154