CGA (HCG-Alpha) & CGB (HCG-Beta) antibody
Reactivity: Human
IHC, StM
Host: Mouse
Monoclonal
HCGab-52
unconjugated
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- Target
- CGA (HCG-Alpha) & CGB (HCG-Beta)
- Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
- Application
- Immunohistochemistry (IHC), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogen
- Purified hCG protein
- Clone
- HCGab-52
- Isotype
- IgG1 kappa
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-
- Application Notes
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Positive Control: JAR or TT cells. Placenta.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
-
- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
-
- Target
- CGA (HCG-Alpha) & CGB (HCG-Beta)
- Background
- This MAb is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the subunit is identical to that of thyroid stimulating hormone (TSH) follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90 % and 60 % of cases respectively. 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
- Molecular Weight
- ~35kDa (intact hCG)
- Gene ID
- 1081, 1082
- UniProt
- P01215, P01233
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