Melanoma Associated Antigen (KBA.62) antibody

Catalog No. ABIN6941354

The Mouse Monoclonal anti-Melanoma Associated Antigen (KBA.62) antibody (Clone KBA-62) (ABIN6941354) specifically detects Melanoma Associated Antigen (KBA.62) in IF and IHC. The antibody is reactive with Human samples.

Quick Overview for Melanoma Associated Antigen (KBA.62) antibody (ABIN6941354)

Target: Melanoma Associated Antigen (KBA.62)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: KBA-62

Product Details

Purpose: KBA.62 (Melanoma Associated Antigen) Antibody

Specificity: KBA.62 is a novel anti-melanoma antibody. It reacts positively against melanocytic tumors but not other tumors, thus demonstrating specificity and sensitivity. Moreover, it reacts positively against junctional nevus cells but not intradermal nevi, and against fetal melanocytes but not normal adult melanocytes. KBA.62 antibody is useful in identifying malignant melanomas. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, sarcomas, spindle cell carcinomas and various types of mesenchymal neoplasms. A keratin-negative, vimentin-rich neoplasm that immuno-reacts with antibody to S100 protein and with KBA.62 antibody is, with rare exception, a melanoma. Anti-KBA.62 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micro-metastasis in sentinel lymph node.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human KAL cells derived from lymph node metastasis of malignant melanoma

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Application: Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Melanoma Associated Antigen (KBA.62)

Alternative Name: KBA.62 (Melanoma Associated Antigen)

Background:

Human Melanoma Associated Antigen, KBA.62

SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Molecular Weight: 135 and 128kDa and two weak bands of 88 and 73kDa)| (140