FITC antibody

Catalog No. ABIN6941359

The Mouse Monoclonal anti-FITC antibody is suitable to detect FITC in samples from Various Species. It has been validated for WB, IHC, FACS and IF.

Quick Overview for FITC antibody (ABIN6941359)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reactivity: Various Species

Host: Mouse

Clonality: Monoclonal

Conjugate: This FITC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SPM395

Product Details anti-FITC Antibody

Purpose: FITC (Fluorescein Isothiocyanate) Antibody

Specificity: This MAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this MAb is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Fluorescein isothiocyanate (FITC) conjugated to KLH

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Fluoresceinated proteins in solution or on tissues.

Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Pretreatment depends upon the fluoreceinated primary antibody.)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC (Fluorescein Isothiocyanate)

Target Type: Chemical

Background:

Fluorescein isothiocyanate, FITC

Fluoresceinated proteins in solution or on tissues.

Molecular Weight: 389.4Da (Molecular Formula: C21H11NO5S)