Macrophage / Histiocytoma Marker antibody

Catalog No. ABIN6941389

This Mouse Monoclonal antibody specifically detects Macrophage / Histiocytoma Marker in IHC and StM. It exhibits reactivity toward Human.

Quick Overview for Macrophage / Histiocytoma Marker antibody (ABIN6941389)

Target: Macrophage / Histiocytoma Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Macrophage / Histiocytoma Marker antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: D11

Product Details anti-Macrophage / Histiocytoma Marker Antibody

Specificity: In Western blotting, it detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

No Cross-Reactivity: Mouse (Murine), Pig (Porcine), Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: Membrane preparation from human hepatocytes

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Liver or Histiocytoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Macrophage / Histiocytoma Marker

Target: Macrophage / Histiocytoma Marker

Alternative Name: Macrophage & Histiocytoma Marker

Molecular Weight: 125kDa -135kDa