MLANA & SILV antibody
The Mouse Monoclonal anti-MLANA & SILV antibody (Clone DT101-BC199-HMB45) (ABIN6941419) specifically detects MLANA & SILV in IHC, ISt and StM.
The antibody is reactive with Human samples.
Catalog No. ABIN6941419
$640.46
Plus shipping costs $50.00
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United States
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Quick Overview for MLANA & SILV antibody (ABIN6941419)
Target
MLANA & SILV
Reactivity
Human
Host
Mouse
Clonality
Monoclonal
Conjugate
Un-conjugated
Application
Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
Clone
DT101-BC199-HMB45
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Specificity
- This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
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Purification
- Purified by Protein A/G
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Immunogen
- Recombinant hMART-1 protein (DT101 & BC199), Extract of pigmented melanoma metastases from lymph nodes (HMB45)
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Isotype
- IgG1 kappa
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Application Notes
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Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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Restrictions
- For Research Use only
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Concentration
- 200 μg/mL
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Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Expiry Date
- 24 months
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