Keratin 77 antibody

Catalog No. ABIN6941429

This anti-Keratin 77 antibody is a Mouse Monoclonal antibody detecting Keratin 77 in IHC, WB, IF, FACS and StM. Suitable for Human, Rat, Cow, Dog, Mouse, Rabbit, Chicken, Monkey and Turtle.

Quick Overview for Keratin 77 antibody (ABIN6941429)

Target: Keratin 77 (KRT77)

Reactivity: Human, Rat, Cow, Dog, Mouse, Rabbit, Chicken, Monkey, Turtle

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 77 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: AE-1

Product Details anti-Keratin 77 Antibody

Specificity: This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Purification: Purified by Protein A/G

Immunogen: Human epidermal keratin

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Skin or squamous cell carcinoma (IHC).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Keratin 77

Target: Keratin 77 (KRT77)

Alternative Name: KRT77

Molecular Weight: 40-56.5kDa

Gene ID: 374454

UniProt: Q7Z794