Recombinant Keratin 77 antibody

Catalog No. ABIN6941453

This anti-Keratin 77 antibody is a Rabbit Monoclonal antibody detecting Keratin 77 in IHC and StM. Suitable for Human.

Quick Overview for Recombinant Keratin 77 antibody (ABIN6941453)

Target: Keratin 77 (KRT77)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Keratin 77 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: KRTL-1577R

Product Details anti-Keratin 77 Antibody

Specificity: This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Cross-Reactivity (Details): Expected to show broad species reactivity.

Purification: Purified by Protein A/G

Immunogen: Recombinant human KRT77 protein fragment

Isotype: IgG

Application Details

Application Notes:

Positive Control: Skin, Squamous cell carcinoma (SCC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Keratin 77

Target: Keratin 77 (KRT77)

Alternative Name: KRT77

Molecular Weight: 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)

Gene ID: 374454

UniProt: Q7Z794