B4GALNT1 antibody (AA 451-533)

Catalog No. ABIN6942198

This Rabbit Polyclonal antibody specifically detects B4GALNT1 in WB, ELISA, IF (cc), IF (p), ICC, IHC (fro) and IHC (p). It exhibits reactivity toward Mouse.

Quick Overview for B4GALNT1 antibody (AA 451-533) (ABIN6942198)

Target: B4GALNT1 (beta-1,4-N-Acetyl-Galactosaminyl Transferase 1 (B4GALNT1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B4GALNT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-B4GALNT1 Antibody

Binding Specificity: AA 451-533

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human B4GALNT1/GM2 synthase

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for B4GALNT1

Target: B4GALNT1 (beta-1,4-N-Acetyl-Galactosaminyl Transferase 1 (B4GALNT1))

Alternative Name: B4GALNT1/GM2 synthase

Background:

Synonyms: (N acetylneuraminyl) galactosylglucosylceramide, (N-acetylneuraminyl)-galactosylglucosylceramide, 4 N-acetylgalactosaminyltransferase 1, 4933429D13Rik, B4GALNT1, B4GN1_HUMAN, Beta 1 4 N acetyl galactosaminyl transferase 1, Beta 1 4 N acetylgalactosaminyltransferase 1, Beta-1, BETA-1,4-N-ACETYLGALACTOSAMINYLTRANSFERASE 1, BETA-1,4-N-ACETYLGALACTOSAMINYLTRANSFERASE, Beta1 4GalNAc T, Gal-NAc-T, GALGT, Galgt1, GalNAc T, GalNAc-T, GALNACT, GD2 synthase, Ggm-2, Ggm2, GM2 synthase, GM2/GD2 synthase, SIAT 2, SIAT2, UDP Gal:betaGlcNAc beta 1 4 N acetylgalactosaminyltransferase transferase 1, UDP N acetyl alpha D galactosamine:(N acetylneuraminyl).

Background: GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]

Gene ID: 2583

UniProt: Q00973