CD3D antibody

Catalog No. ABIN6942432

This Rabbit Monoclonal antibody specifically detects CD3D in WB, IHC, FACS, IHC (p) and IF (cc). It exhibits reactivity toward Human.

Quick Overview for CD3D antibody (ABIN6942432)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD3D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 5C1

Product Details anti-CD3D Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: C terminus human CD3D

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Alternative Name: CD3D

Background:

Synonyms: T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD_antigen: CD3d, CD3D

Background: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells(PubMed:12215456).

Gene ID: 915

UniProt: P04234

Pathways: TCR Signaling, CXCR4-mediated Signaling Events