CD8 alpha antibody

Catalog No. ABIN6942455

This anti-CD8 alpha antibody is a Rabbit Monoclonal antibody detecting CD8 alpha in IHC, WB and IF (cc). Suitable for Human.

Quick Overview for CD8 alpha antibody (ABIN6942455)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 72B1

Product Details anti-CD8 alpha Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Recombinant human CD8 alpha near C-terminus

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8 alpha

Background:

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD_antigen: CD8a, CD8A, MAL

Background: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Gene ID: 925

UniProt: P01732

Pathways: TCR Signaling