CLOCK antibody

Catalog No. ABIN6942585

This Rabbit Monoclonal antibody specifically detects CLOCK in WB, IHC (p), FACS, IF (cc) and IF (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CLOCK antibody (ABIN6942585)

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CLOCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 3F9

Product Details anti-CLOCK Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CLOCK

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Alternative Name: CLOCK

Background:

Synonyms: Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, hCLOCK, bHLHe8, CLOCK, BHLHE8, KIAA0334, clock circadian regulator

Background: Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. Clock, a member of the Basic-helix-loop-helix-psp (bHLH-PAS) family of transcription factors, has also been identified as having circadian function. Mutations within the clock gene have been shown to increase the length of the endogenous period and To contain a loss of rhythmicity of circadian oscillations. Clock contains a DNA-binding domain, a protein dimerization domain and a glutamine-rich C-terminal region, which indicates transactivation ability. It has been speculated that Clock may regulation circadian rhythmicity in combination with Other proteins such as Per. Per is also a PAS-domain containing protein that exhibits circadian function. Highest expression of Clock is seen in the hypothalamus and the eye.

Gene ID: 9575

UniProt: O15516

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism