EN1 antibody (AA 351-392) (AbBy Fluor® 750)

Catalog No. ABIN6943252

The Rabbit Polyclonal anti-EN1 antibody has been validated for WB, FACS, IF (cc), IF (p). It is suitable to detect EN1 in samples from Human, Mouse, Rat.

Quick Overview for EN1 antibody (AA 351-392) (AbBy Fluor® 750) (ABIN6943252)

Target: EN1 (Engrailed Homeobox 1 (EN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EN1 antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-EN1 Antibody (AbBy Fluor® 750)

Binding Specificity: AA 351-392

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EN1/Engrailed 1

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EN1

Target: EN1 (Engrailed Homeobox 1 (EN1))

Alternative Name: EN1/Engrailed 1

Background:

Synonyms: En 1, EN1, Engrailed 1, Engrailed, Engrailed homeobox 1, Engrailed homolog 1, HME1_HUMAN, Homeobox protein en-1, Homeobox protein engrailed 1, Homeobox protein engrailed-1, Hu-En-1, Mo-en.1.

Background: The engrailed-1 gene, EN1, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation. In Drosophila, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homo-logs, EN-1 and EN-2, produce different developmental defects that frequently are lethal. EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum. EN-1 and EN-2 regulate expres-sion of a-synuclein, a gene that is genetically linked to Parkinson?s disease.

Gene ID: 2019

UniProt: Q05925

Pathways: Dopaminergic Neurogenesis