EPAS1 antibody

Catalog No. ABIN6943257

This Rabbit Monoclonal antibody specifically detects EPAS1 in WB, FACS and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for EPAS1 antibody (ABIN6943257)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2G3

Product Details anti-EPAS1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant full length Human protein

Isotype: IgG

Application Details

Application Notes: WB(1:500-2000), FCM(1:50-100), IHC-P(1:50-200)

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: EPAS1

Background:

Synonyms: HIF-2 alpha, Endothelial PAS domain-containing protein 1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, HIF-1-alpha-like factor, Hypoxia-inducible factor 2-alpha, PAS domain-containing protein 2, EPAS-1, bHLHe73, HLF, HIF2-alpha, EPAS1, BHLHE73, HIF2A, MOP2, PASD2, ECYT4

Background: Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT, heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

Gene ID: 2034

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect