gamma Tubulin antibody

Catalog No. ABIN6943513

The Mouse Monoclonal anti-gamma Tubulin antibody has been validated for IF (p), WB and IHC (p). It is suitable to detect gamma Tubulin in samples from Mouse, Human and Rat.

Quick Overview for gamma Tubulin antibody (ABIN6943513)

Target: gamma Tubulin (TUBG) (Tubulin, gamma (TUBG))

Reactivity: Mouse, Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This gamma Tubulin antibody is un-conjugated

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 8D11

Product Details anti-gamma Tubulin Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G.

Immunogen: KLH conjugated synthetic peptide derived from human Gamma Tubulin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for gamma Tubulin

Target: gamma Tubulin (TUBG) (Tubulin, gamma (TUBG))

Alternative Name: Gamma Tubulin

Background:

Synonyms: Centrosome Marker, Gamma 1 tubulin, gamma tubulin, gamma-tubulin, Gamma 2 tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma tubulin complex component 1, GCP 1, GCP-1, GCP1, MGC131994, TUBG, TUBG1, TUBG2, Tubulin gamma 1 chain, Tubulin gamma 2 chain, Xgam, TBG1_HUMAN, TBG2_HUMAN, Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1.

Background: Centrosome Marker
Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end.
The abundance of Gamma tubulin is less than 1 % of the level of either alpha or beta tubulin. It shares approximately 28-32 % identity with alpha tubulin from various organisms and 32-36 % identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.

Gene ID: 7283

UniProt: P23258