KARS antibody

Catalog No. ABIN6943858

The Rabbit Monoclonal anti-KARS antibody has been validated for WB, IP and IHC (p). It is suitable to detect KARS in samples from Human, Mouse and Rat.

Quick Overview for KARS antibody (ABIN6943858)

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KARS antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1H8

Product Details anti-KARS Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein corresponding to C-terminal human Lysyl tRNA synthetase.

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IP(1-2 μg)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for KARS

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Alternative Name: KARS

Background:

Synonyms: Lysine tRNA ligase, Lysyl tRNA synthetase, LysRS, KARS, KIAA0070

Background: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.

Gene ID: 3735

UniProt: Q15046