LIG4 antibody

Catalog No. ABIN6943923

This Rabbit Monoclonal antibody specifically detects LIG4 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for LIG4 antibody (ABIN6943923)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This LIG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2D9

Product Details anti-LIG4 Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human DNA Ligase IV aa 500-700

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Alternative Name: LIG4

Background:

Synonyms: DNA ligase 4, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4, LIG4.

Background: Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Gene ID: 3981

UniProt: P49917

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response