MAD2L1 antibody

Catalog No. ABIN6943982

This anti-MAD2L1 antibody is a Rabbit Monoclonal antibody detecting MAD2L1 in WB, IF (cc), IF (p) and IHC (p). Suitable for Human and Mouse.

Quick Overview for MAD2L1 antibody (ABIN6943982)

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MAD2L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 6C6

Product Details anti-MAD2L1 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human Mad2L1 aa 1-150

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for MAD2L1

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Alternative Name: Mad2L1

Background:

Synonyms: Mitotic spindle assembly checkpoint protein MAD2A, Mitotic arrest deficient 2-like protein 1, HsMAD2, MAD2-like protein 1, MAD2L1, MAD2.

Background: Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T47D, was shown to complete mitosis in the presence of nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during prometaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with insulin receptor (IR), but not IGFIR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and if the process is not complete, MAD2B delays the onset of anaphase.

Gene ID: 4085

UniProt: Q13257