Prohibitin antibody

Catalog No. ABIN6944678

This anti-Prohibitin antibody is a Rabbit Monoclonal antibody detecting Prohibitin in WB, FACS, IHC (p), IF (cc) and IHC (fro). Suitable for Human, Mouse, Rat, Zebrafish (Danio rerio), Crucian Carp and Fish.

Quick Overview for Prohibitin antibody (ABIN6944678)

Target: Prohibitin (PHB)

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Crucian Carp, Fish

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1F10

Product Details anti-Prohibitin Antibody

Cross-Reactivity: Fish, Human, Mouse, Rat, Zebrafish (Danio rerio)

Cross-Reactivity (Details): Crucian carp (Carassius carassius)

Purification: Purified by Protein A.

Immunogen: N terminal Human Prohibitin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: Prohibitin

Background:

Synonyms: Prohibitin, PHB

Background: Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and protein levels are high in G1, decline during the S phase, rise again in G2 and decline in M phase, which suggests that prohibitin controls the cell cycle by using both transcriptional and posttranslational mechanisms. Prohibitin is also a potential tumor suppressor protein that binds to retinoblastoma (Rb) and subsequently inhibits the activity of E2F family members in response to specific signaling cascades. Prohibitin 2 is a repressor of estrogen receptor activity, and is required for somatic and germline differentiation in the larval gonad during embryonic development. Mutations in the Prohibitin genes are correlated with breast cancer development and/or progressionin more than 80 % of the cell lines analyzed.

Gene ID: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling