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Prohibitin antibody (AA 167-261)

PHB Reactivity: Human WB, IHC, IF, StM Host: Mouse Monoclonal PHB-3231 unconjugated
Catalog No. ABIN6940301
  • Target See all Prohibitin (PHB) Antibodies
    Prohibitin (PHB)
    Binding Specificity
    • 16
    • 13
    • 12
    • 11
    • 9
    • 9
    • 9
    • 8
    • 8
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 167-261
    Reactivity
    • 136
    • 62
    • 60
    • 11
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 116
    • 20
    Mouse
    Clonality
    • 105
    • 31
    Monoclonal
    Conjugate
    • 69
    • 12
    • 8
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Prohibitin antibody is un-conjugated
    Application
    • 104
    • 51
    • 45
    • 27
    • 22
    • 21
    • 14
    • 13
    • 11
    • 9
    • 6
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)
    Specificity
    Recognizes a protein of 30 kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant human PHB protein fragment (aa167-261) (exact sequence is proprietary)
    Clone
    PHB-3231
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product PHB Primary Antibody
  • Application Notes

    Positive Control: Ramos, Jurkat, HEK293, K562, HepG2, A431 or MCF-7 cells (IF). Liver, Kidney, Heart or Endometrium (IHC).

    Known Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Prohibitin (PHB)
    Alternative Name
    PHB (PHB Products)
    Molecular Weight
    30kDa
    Gene ID
    5245
    UniProt
    P35232
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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