Splicing Factor 1 antibody (pSer82)

Catalog No. ABIN6945041

This anti-Splicing Factor 1 antibody is a Rabbit Polyclonal antibody detecting Splicing Factor 1 in WB, ELISA, IHC (p), IF (cc), IF (p), ICC and IHC (fro). Suitable for Mouse.

Quick Overview for Splicing Factor 1 antibody (pSer82) (ABIN6945041)

Target: Splicing Factor 1 (SF1)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Splicing Factor 1 Antibody

Binding Specificity: pSer82

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human splicing factor 1 around the phosphorylation site of Ser82

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: Splicing factor 1

Background:

Synonyms: splicing factor 1 (phospho S82), p-splicing factor 1 (phospho S82), BBP, D11S636, Mammalian branch point-binding protein mBBP, OTTHUMP00000069749, OTTHUMP00000069750, OTTHUMP00000069752, splicing factor 1, Transcription factor ZFM1, ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, ZNF162.

Background: This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Gene ID: 7536

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location