UBA1 antibody

Catalog No. ABIN6945248

The Rabbit Monoclonal anti-UBA1 antibody (Clone 8A4) (ABIN6945248) specifically detects UBA1 in WB, IHC, FACS, IF (cc) and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for UBA1 antibody (ABIN6945248)

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This UBA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 8A4

Product Details anti-UBA1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Human Ubiquitin-like modifier-activating enzyme 1 between 1000 amino acids to C-terminus

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for UBA1

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Alternative Name: Ubiquitin-like modifier-activating enzyme 1

Background:

Synonyms: Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1

Background: Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).

Gene ID: 7317

UniProt: P22314