Histone H3.3 antibody

Catalog No. ABIN6952331

This Rabbit Monoclonal antibody specifically detects Histone H3.3 in WB, ChIP and ChIP-seq. It exhibits reactivity toward Human.

Quick Overview for Histone H3.3 antibody (ABIN6952331)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone H3.3 Antibody

Specificity: Monoclonal antibody raised in rabbit against histone variant H3.3, using a KLH-conjugated synthetic peptide.

Purification: Protein A purified

Immunogen: synthetic peptide

Application Details

Application Notes: ChIP/ChIP-seq 1 μg:ChIP
WB 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: PBS, 50 % glycerol, 1 % BSA , 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: Histone H3.3

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Histone variant H3.3 is preferably present at active genes.

UniProt: P84243