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Histone 3 antibody (H3K27me3)

ChIP-seq Grade H3 Reactivity: Human WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, SNAP-ChIP Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6952339
  • Target See all Histone 3 (H3) Antibodies
    Histone 3 (H3)
    Binding Specificity
    • 60
    • 47
    • 45
    • 42
    • 41
    • 37
    • 36
    • 35
    • 35
    • 35
    • 34
    • 32
    • 31
    • 30
    • 29
    • 29
    • 26
    • 25
    • 23
    • 23
    • 23
    • 22
    • 20
    • 18
    • 18
    • 17
    • 17
    • 16
    • 16
    • 16
    • 16
    • 16
    • 15
    • 14
    • 14
    • 13
    • 13
    • 12
    • 12
    • 12
    • 12
    • 12
    • 11
    • 11
    • 10
    • 10
    • 10
    • 9
    • 9
    • 8
    H3K27me3
    Reactivity
    • 1402
    • 941
    • 861
    • 59
    • 42
    • 41
    • 41
    • 40
    • 39
    • 35
    • 35
    • 30
    • 29
    • 22
    • 19
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 1219
    • 244
    • 8
    • 4
    Rabbit
    Clonality
    • 1120
    • 354
    • 1
    Polyclonal
    Conjugate
    • 824
    • 81
    • 63
    • 63
    • 63
    • 62
    • 62
    • 62
    • 37
    • 33
    • 23
    • 17
    • 17
    • 17
    • 17
    • 17
    • 14
    • 2
    • 1
    This Histone 3 antibody is un-conjugated
    Application
    • 1061
    • 410
    • 381
    • 316
    • 253
    • 188
    • 181
    • 180
    • 163
    • 160
    • 142
    • 126
    • 119
    • 48
    • 38
    • 34
    • 32
    • 20
    • 15
    • 14
    • 7
    • 7
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Sample Normalization and Antibody Profiling ChIP (SNAP-ChIP)
    Specificity
    Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 27 (H3K27me3), using a KLH-conjugated synthetic peptide.
    Cross-Reactivity
    Arabidopsis, Caenorhabditis elegans (C. elegans), Daphnia, Fruit Fly (Drosophila melanogaster), Maize/Corn (Zea mays), Mouse (Murine), Poplar (Populus), Red Algae (Chondrus), Tomato (Solanum lycopersicum)
    Cross-Reactivity (Details)
    wide range
    Purification
    Peptide affinity purified
    Grade
    ChIP-seq Grade
    Immunogen
    synthetic peptide
    Top Product
    Discover our top product H3 Primary Antibody
  • Application Notes
    ChIP/ChIP-seq 1 - 2 μg:ChIP
    ELISA 1:100 - 1:500
    Dot Blot 1:5,000
    WB 1:1,000
    IF 1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.1 μg/μL
    Buffer
    PBS, 0.05 % azide, 0.05 % ProClin300
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Wang, Zhao, Liu, Wang, Qiao, Yi, Jiang, Kou, Zhao, Yin, Li, Wang, Jiang, Gao, Chen: "BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding." in: Protein & cell, (2022) (PubMed).

  • Target
    Histone 3 (H3)
    Alternative Name
    Histone 3 (H3 Products)
    Synonyms
    H-3 antibody, histocompatibility 3 antibody, H3 antibody
    Background
    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K27 is associated with inactive genomic regions.
    UniProt
    P68431
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