Histone 3 antibody (H3K27me3)

Catalog No. ABIN6952339

This anti-Histone 3 antibody is a Rabbit Polyclonal antibody detecting Histone 3 in WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN and SNAP-ChIP. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for Histone 3 antibody (H3K27me3) (ABIN6952339)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Sample Normalization and Antibody Profiling ChIP (SNAP-ChIP)

Grade: ChIP-seq Grade

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K27me3

Specificity: Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 27 (H3K27me3), using a KLH-conjugated synthetic peptide.

Cross-Reactivity: Arabidopsis, Caenorhabditis elegans (C. elegans), Daphnia, Fruit Fly (Drosophila melanogaster), Maize/Corn (Zea mays), Mouse (Murine), Poplar (Populus), Red Algae (Chondrus), Tomato (Solanum lycopersicum)

Cross-Reactivity (Details): wide range

Purification: Peptide affinity purified

Immunogen: synthetic peptide

Application Details

Application Notes: ChIP/ChIP-seq 1 - 2 μg:ChIP
ELISA 1:100 - 1:500
Dot Blot 1:5,000
WB 1:1,000
IF 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 μg/μL

Buffer: PBS, 0.05 % azide, 0.05 % ProClin300

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-Histone 3 antibody (ABIN6952339)

Wang, Zhao, Liu, Wang, Qiao, Yi, Jiang, Kou, Zhao, Yin, Li, Wang, Jiang, Gao, Chen: "BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding." in: Protein & cell, (2022) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone 3

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K27 is associated with inactive genomic regions.

UniProt: P68431