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Histone 3 antibody (H3K4me3)

ChIP-seq Grade H3 Reactivity: Human WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag, PeA, SNAP-ChIP Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6952351
  • Target See all Histone 3 (H3) Antibodies
    Histone 3 (H3)
    Binding Specificity
    • 56
    • 47
    • 44
    • 41
    • 40
    • 36
    • 35
    • 35
    • 34
    • 34
    • 34
    • 32
    • 29
    • 29
    • 28
    • 26
    • 26
    • 25
    • 23
    • 22
    • 22
    • 22
    • 20
    • 18
    • 18
    • 17
    • 16
    • 16
    • 16
    • 16
    • 16
    • 16
    • 15
    • 14
    • 14
    • 13
    • 13
    • 12
    • 12
    • 12
    • 11
    • 11
    • 11
    • 10
    • 10
    • 10
    • 9
    • 9
    • 9
    • 8
    H3K4me3
    Reactivity
    • 1368
    • 938
    • 860
    • 59
    • 42
    • 41
    • 41
    • 40
    • 39
    • 35
    • 35
    • 29
    • 26
    • 22
    • 19
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 1184
    • 241
    • 8
    • 4
    Rabbit
    Clonality
    • 1108
    • 328
    • 1
    Polyclonal
    Conjugate
    • 795
    • 81
    • 45
    • 45
    • 45
    • 45
    • 45
    • 45
    • 34
    • 33
    • 21
    • 18
    • 18
    • 18
    • 17
    • 17
    • 17
    • 17
    • 17
    • 17
    • 17
    • 17
    • 12
    • 1
    This Histone 3 antibody is un-conjugated
    Application
    • 1035
    • 402
    • 372
    • 312
    • 238
    • 188
    • 175
    • 166
    • 163
    • 150
    • 138
    • 126
    • 117
    • 48
    • 38
    • 34
    • 32
    • 20
    • 15
    • 13
    • 7
    • 6
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Peptide Array (PeA), Sample Normalization and Antibody Profiling ChIP (SNAP-ChIP)
    Specificity
    Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 4 (H3K4me3), using a KLH-conjugated synthetic peptide.
    Cross-Reactivity
    Arabidopsis, Daphnia, Maize/Corn (Zea mays), Mouse (Murine), Poplar (Populus), Rice (Oryza sativa), Tomato (Solanum lycopersicum), Trout, Zebrafish (Danio rerio)
    Cross-Reactivity (Details)
    wide range
    Purification
    Peptide affinity purified
    Grade
    ChIP-seq Grade
    Immunogen
    synthetic peptide
    Top Product
    Discover our top product H3 Primary Antibody
  • Application Notes
    ChIP/ChIP-seq<1 μg:ChIP
    ELISA 1:1,000
    Dot Blot 1:10,000
    Peptde Array 1:10,000
    WB 1:1,000
    IF 1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.3 μg/μL
    Buffer
    PBS, 0.05 % azide, 0.05 % ProClin300
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Schreiber, Mercier, Jiménez, Ye, García-Sánchez, Klein, Meunier, Ghimire, Birck, Jost, de Lichtenberg, Honoré, Serup, Gradwohl: "Extensive NEUROG3 occupancy in the human pancreatic endocrine gene regulatory network." in: Molecular metabolism, Vol. 53, pp. 101313, (2021) (PubMed).

  • Target
    Histone 3 (H3)
    Alternative Name
    Histone 3 (H3 Products)
    Synonyms
    H-3 antibody, histocompatibility 3 antibody, H3 antibody
    Background
    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K4 is associated with activation of gene transcription.
    UniProt
    P68431
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