SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) (AA 1-422) antibody

Catalog No. ABIN7072836

This Rabbit Monoclonal antibody specifically detects in ELISA, WB, IF, FACS, ICC, IHC (p) and IP. It exhibits reactivity toward Coronavirus (CoV).

Quick Overview for SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) (AA 1-422) antibody (ABIN7072836)

Target: SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N)

Reactivity: Coronavirus (CoV)

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details

Binding Specificity: AA 1-422

Characteristics: 2019-nCoV CoV Nucleocapsid Has cross-reactivity in ELISA and WB with SARS-CoV Nucleoprotein / NP Protein .

Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant SARS-CoV Nucleoprotein / NP ( NP_828858.1, Met1-Ala422).

Application Details

Application Notes: WB 1:1000-1:5000
Validated Applications:WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)

Restrictions: For Research Use only

Handling

Buffer: 0.2 um filtered solution in PBS

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Target Details

Target: SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N)

Alternative Name: Coronavirus Nucleocapsid Protein

Target Type: Viral Protein

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.